Rebecca Root was born a boy. She trained as an actor at Mountview Academy of Theatre Arts in London. When she was in her thirties, she adopted a female name and lived as a woman before having gender reassignment surgery. Her acting career has spanned television, film, theatre and radio. In 2015 she starred in the BBC series Boy Meets Girl, and became the first transgender actor to be cast in a lead transgender role in a mainstream UK sitcom. She received an Attitude Award for her performance.
Root also appears in the film The Danish Girl with Eddie Redmayne. Alongside her acting career, she works as a voice and speech teacher, specialising in vocal adaptation for trans clients.